Mansoureh Sedaghati,; Assareh Mohammad Hasan
Abstract
It is essential to use corrective techniques in the cultivation of medicinal plants and to improve the quantity and quality of the essential oils and essence in them for the production ...
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It is essential to use corrective techniques in the cultivation of medicinal plants and to improve the quantity and quality of the essential oils and essence in them for the production of high-yielding plants. The influence factors in callus production and regeneration include genotype, growth regulators, culture medium, age and type of explants and environmental conditions. In this study, callus induction in seven plant species (Daucus carota, Dracocephalum kotschyi, Hyssopus officinalis, Galega officinalis, Amsonia tubemaemontana, Moringa oliefera, Urtica dioica) has been investigated in vitro, which were evaluated callus induction and organogenesis on the leafy specimen in MS and WPM medium with different concentrations of growth regulators. For this purpose, a factorial experiment was designed in a completely randomized design (CRD) and was investigated genotypic effects on callus induction and subsequent proliferation of the plant. The results showed that Daucus carota, Moringa oliefera and Hyssopus officinalis had a significant difference in callus induction with other species.
